![]() This article was selected as a Paper of the Week. Section 1734 solely to indicate this fact. This article must therefore be hereby marked “ advertisement” in accordance with 18 U.S.C. The costs of publication of this article were defrayed in part by the payment of page charges. This work was also supported by the Oregon State University College of Pharmacy. This work was supported, in whole or in part, by National Institutes of Health Grant GM60852 (to M. The abbreviations used are: NCoR, nuclear receptor corepressor COUP-TF, chicken ovalbumin upstream promoter transcription factor SUMO, small ubiquitin-like modifier TNFα, tumor necrosis factor α HA, hemagglutinin GST, glutathione S-transferase MS, mass spectrometry MS/MS, tandem mass spectrometry CAT, chloramphenicol acetyltransferase ChIP, chromatin immunoprecipitation siRNA, small interfering RNA CHX, cycloheximide co-IP, co-immunoprecipitation RD, repression domain DBD, DNA-binding domain RT-qPCR, quantitative reverse transcription-PCR. Together, these studies identify a novel COUP-TFI complex that functions as a repressor of transcription and may play a role in the TNFα signaling pathways. Finally, our studies highlight a central role for COUP-TFI in the induction of the TNFAIP8 promoter by TNFα. The gene encoding the anti-apoptotic protein TNFAIP8 ( tumor necrosis factor α (TNFα)- induced protein 8) was identified as being repressed by COUP-TFI in a manner that required several of the component proteins of the COUP-TFI complex. DBC1 stabilized the interaction between COUP-TFI and NCoR by interacting directly with both proteins. In vitro experiments revealed that COUP-TFI interacted directly with NCoR but in a manner different from that of other nuclear receptors. The proapoptotic protein DBC1 was also identified in COUP-TFI complexes. A tandem affinity purification procedure revealed that COUP-TFI associated with a number of transcriptional regulatory proteins in HeLa S3 cells, including the nuclear receptor corepressor (NCoR), TIF1β/KAP-1, HDAC1, and the SWI/SNF family member Brahma. THE BIG DEAL: One project is selected for (up to) $1 Million in production financing and a release in Cineplex Theatres.The orphan nuclear receptor chicken ovalbumin upstream promoter transcription factor I (COUP-TFI) plays key roles in development and homeostasis.GIVE THE ULTIMATE PITCH: Before a live audience, pitch your project to a panel of industry pros and convince them that yours is the project that Canada wants to see.GATEWAY 5: From 15 to down to the Final 5.GATEWAY 4: From 30 to down to the Top 15.GATEWAY 3: From 60 to down to the Top 30.GATEWAY 2: From 120 to down to the Top 60.GET THOSE FANS TO SUPPORT YOU: Get your fans to help you advance by sharing your completed missions over social media, spreading your awesomeness, and voting for you through three designated voting windows.BUILD FANS: Build your fan base by promoting your projects through social media (such as Facebook and Twitter).CREATE CONTENT: Execute weekly “missions” designed to develop and market your project. ![]() ADD SOCIAL ACCOUNTS: Create unique Facebook and Twitter accounts for your project.SUBMIT A TRAILER: Submit a 60 second movie trailer that showcases your film concept.SIGN UP: Sign up with a team of three Canadians who have a combined talent for filmmaking, marketing and social engagement.
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